分子量:27.7 kDa

纯度:>92% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg of the Human Kallikrein 3, His Tag by the LAL method.

Buffer:50 mM Tris, 150 mM NaCl,  pH 7.5

生物活性:Measured by its ability to cleave the colorimetric peptide substrate, Succinyl  Arg  Pro  Tyr  p  Nitroanilide (Suc-RPY-pNA). The specific activity is >60 pmoles / min / μg.

产品特性:Human Kallikrein 3, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 27.7 kDa. The predicted N-terminus is Ala 18. The reducing (R) protein migrates as 36 kDa in SDS-PAGE  due to glycosylation.

产品背景:Kallikrein-3 (KLK3) is also known as Prostate-specific antigen (PSA), APS, Gamma-seminoprotein (Seminin), P-30 antigen, Semenogelase, is a glycoprotein enzyme, is a member of the kallikrein-related peptidase family and is secreted by the epithelial cells of the prostate gland. The preferential cleavage site of KLK3 is “-Tyr-|-Xaa-“, the catalytic activity is inhibited by SERPINA5 and is strongly inhibited by Zn2+. KLK3 hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.