分子量:42 kDa

纯度:>95% as determined by reduced SDS-PAGE. 

内毒素:Less than 1.0 EU per μg of the Biotinylated Human CTLA-4, Fc tag by the LAL method.

Buffer:50 mM tris, 100 mM glycine, pH7.5

生物活性:

产品特性:This protein carries a a 15 AA fragment ,containing one single lysine residue that is enzymatically labeled with biotin in vivo, at the C-terminus, followed by a human IgG1 Fc fragment. The protein has a calculated MW of 42 kDa. The predicted N-terminus is Ala 37. The reducing (R) protein migrates as 50-55 kDa in SDS-PAGE due to glycosylation.

产品背景:CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4) is also known as CD152 (Cluster of differentiation 152), is a protein receptor that downregulates the immune system. CTLA4 is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. The protein contains an extracellular V domain, a transmembrane domain, and a cytoplasmic tail. Alternate splice variants, encoding different isoforms. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function. Fusion proteins of CTLA4 and antibodies (CTLA4-Ig) have been used in clinical trials for rheumatoid arthritis.